Anticorps Polyclonal de lapin anti-GP repeat
GP repeat Polyclonal Antibody for WB, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (1)
Applications
WB, IHC, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 24494-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | Protéine recombinante | 
| Résultats positifs en IHC | tissu de cervelet humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| Immunohistochimie (IHC) | IHC : 1:200-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 9 publications below | 
| IHC | See 5 publications below | 
| IF | See 6 publications below | 
| ELISA | See 1 publications below | 
Informations sur le produit
24494-1-AP cible GP repeat dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain | 
| Réactivité citée | Humain, souris | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Polyclonal | 
| Type | Anticorps | 
| Immunogène | Peptide | 
| Nom complet | GP repeat | 
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par affinité contre l'antigène | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GP repeated sequence. The recombinant protein 6*His tag-SMARCA4 or GST tag-SMARCA4 which contains the sequence "GPGPGPGPG" were used to detect this antibody.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for GP repeat antibody 24494-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Nucleic Acids Res High-throughput screen of 100 000 small molecules in C9ORF72 ALS neurons identifies spliceosome modulators that mobilize G4C2 repeat RNA into nuclear export and repeat associated non-canonical translation | ||
| Acta Neuropathol Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers. | ||
| Acta Neuropathol Increased prevalence of granulovacuolar degeneration in C9orf72 mutation. | ||
| Acta Neuropathol Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. | ||
| EMBO Rep Glucose hypometabolism prompts RAN translation and exacerbates C9orf72-related ALS/FTD phenotypes | ||
| Neuropathol Appl Neurobiol Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins. | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Barbara (Verified Customer) (01-14-2019) | 
 | 

