Anticorps Recombinant de lapin anti-GP73/GOLPH2

GP73/GOLPH2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1I12

N° de cat : 81893-1-PBS

Synonymes

GOLM1, GOLPH2, GP73, 1I12, C9orf155

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Galerie de données de validation

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  WB analysis using 81893-1-RR (same clone as 81893-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81893-1-RR (same clone as 81893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 81893-1-RR (same clone as 81893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81893-1-RR (same clone as 81893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 81893-1-RR (same clone as 81893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:450 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81893-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81893-1-RR against Human GP73/GOLPH2 were performed. The affinity constant is 0.828 nM.

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Informations sur le produit

81893-1-PBS cible GP73/GOLPH2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GP73/GOLPH2 Protéine recombinante Ag7207
• Nom complet: golgi membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC001740
• Symbole du gène: GP73
• Identification du gène (NCBI): 51280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

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