Anticorps Polyclonal de lapin anti-GPAA1

GPAA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10104-1-AP

Synonymes

GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

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Galerie de données de validation

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  WB analysis using 10104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 10104-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10104-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules HepG2, cellules MCF-7, cellules MKN-45, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10104-1-AP cible GPAA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPAA1 Protéine recombinante Ag0152
• Nom complet: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 60-68 kDa
• Numéro d’acquisition GenBank: BC004129
• Symbole du gène: GPAA1
• Identification du gène (NCBI): 8733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

Publications

Species	Application	Title
human	IHC	Mod Pathol; Profiling the expression pattern of GPI transamidase complex subunits in human cancer.; Authors - Nagpal Jatin K JK
human	WB,IHC	Liver Res; Single-cell and machine learning approaches uncover intrinsic immune-evasion genes in the prognosis of hepatocellular carcinoma; Authors - Jiani Wang
mouse	IF	Nat Commun; CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection; Authors - B Vijayalakshmi Ayyar
human	WB	Cell Rep; Targeting the GPI transamidase subunit GPAA1 abrogates the CD24 immune checkpoint in ovarian cancer; Authors - Alok K Mishra ; KD Validated

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