Anticorps Polyclonal de lapin anti-GPAA1

GPAA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10104-1-PBS

Synonymes

GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

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Galerie de données de validation

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  WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

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  WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10104-1-PBS cible GPAA1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPAA1 Protéine recombinante Ag0152
• Nom complet: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 60-68 kDa
• Numéro d’acquisition GenBank: BC004129
• Symbole du gène: GPAA1
• Identification du gène (NCBI): 8733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

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