Anticorps Recombinant de lapin anti-GPAA1

GPAA1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241591C4

N° de cat : 84632-1-RR

Synonymes

241591C4, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

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Galerie de données de validation

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  WB analysis using 84632-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84632-1-RR (GPAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84632-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84632-1-RR against Human GPAA1 were performed. The affinity constant is 0.141 nM.

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Applications testées

• Résultats positifs en WB: cellules MKN-45, cellules HeLa, cellules HepG2, cellules HuH-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84632-1-RR cible GPAA1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPAA1 Protéine recombinante Ag0152
• Nom complet: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 60-68 kDa
• Numéro d’acquisition GenBank: BC004129
• Symbole du gène: GPAA1
• Identification du gène (NCBI): 8733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837).

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