Anticorps Recombinant de lapin anti-GPAM

GPAM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241144H6

N° de cat : 84054-5-PBS

Synonymes

241144H6, EC:2.3.1.15, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT 1, GPAT1

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Galerie de données de validation

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  WB analysis using 84054-5-RR (same clone as 84054-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84054-5-RR (GPAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84054-5-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84054-5-RR (same clone as 84054-5-PBS)

  WB result of GPAM antibody (84054-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPAM transfected HepG2 cells. This data was developed using the same antibody clone with 84054-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84054-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 84054-5-PBS against Human GPAM was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 84054-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84054-5-RR against Human GPAM were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84054-5-PBS cible GPAM dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPAM Protéine recombinante Ag3120
• Nom complet: glycerol-3-phosphate acyltransferase, mitochondrial
• Masse moléculaire calculée: 828 aa, 94 kDa
• Poids moléculaire observé: 90-92 kDa
• Numéro d’acquisition GenBank: BC030783
• Symbole du gène: GPAM
• Identification du gène (NCBI): 57678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.

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