Anticorps Recombinant de lapin anti-GPAM

GPAM Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241144H6

N° de cat : 84054-5-RR

Synonymes

GPAT-1, GPAT1, GPAT 1, Glycerol-3-phosphate acyltransferase 1, mitochondrial, EC:2.3.1.15

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Galerie de données de validation

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  WB analysis using 84054-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84054-5-RR (GPAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 84054-5-RR

  WB result of GPAM antibody (84054-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPAM transfected HepG2 cells.

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  Affinity and Kinetic Characterization of 84054-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84054-5-RR against Human GPAM were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, tissu hépatique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84054-5-RR cible GPAM dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPAM Protéine recombinante Ag3120
• Nom complet: glycerol-3-phosphate acyltransferase, mitochondrial
• Masse moléculaire calculée: 828 aa, 94 kDa
• Poids moléculaire observé: 90-92 kDa
• Numéro d’acquisition GenBank: BC030783
• Symbole du gène: GPAM
• Identification du gène (NCBI): 57678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.

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