Anticorps Polyclonal de lapin anti-Glypican 1

• Phare
• Validé par KD/KO

Glypican 1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 16700-1-AP

Synonymes

glypican, Glypican 1, glypican1, GPC1

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Galerie de données de validation

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  WB analysis of HepG2 using 16700-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16700-1-AP (Glypican 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of BxPC-3 using 16700-1-AP

  IP result of anti-Glypican 1 (IP:16700-1-AP, 4ug; Detection:16700-1-AP 1:600) with BxPC-3 cells lysate 3100 ug.

• 

  IHC staining of human pancreas cancer using 16700-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16700-1-AP (Glypican 1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16700-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16700-1-AP (Glypican 1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2,
• Résultats positifs en IP: cellules BxPC-3,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

16700-1-AP cible Glypican 1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glypican 1 Protéine recombinante Ag10095
• Nom complet: glypican 1
• Masse moléculaire calculée: 558 aa, 62 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC051279
• Symbole du gène: GPC1
• Identification du gène (NCBI): 2817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glypicans are a family of HSPGs that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. Glypicans are considered to have the ability to modulate the activities of HBGFs. The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.

Publications

Species	Application	Title
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
mouse	WB	Matrix Biol; miR-324-5p is up regulated in end-stage osteoarthritis and regulates Indian Hedgehog signalling by differing mechanisms in human and mouse.; Authors - Steven Woods ; KD Validated
human	IHC	Mod Pathol; Glypican-1 immunohistochemistry does not separate mesothelioma from pulmonary adenocarcinoma.; Authors - Kenrry Chiu
human	IHC	Mod Pathol; Glypican-1 immunohistochemistry is a novel marker to differentiate epithelioid mesothelioma from lung adenocarcinoma.; Authors - Vishwa Jeet Amatya
human	WB,IHC,CoIP	Front Cell Dev Biol; The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis.; Authors - Charlie Colin-Pierre
human	IHC	Transl Lung Cancer Res; Glypican-1 is a novel immunohistochemical marker to differentiate poorly differentiated squamous cell carcinoma from solid predominant adenocarcinoma of the lung.; Authors - Yuichiro Kai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vishwa Jeet (Verified Customer) (11-09-2017)

Recently, our publication has been accepted in Modern Pathology.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: x100
• Cell Tissue Type: Lung, Mesothelioma

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