Anticorps Recombinant de lapin anti-GPC1

GPC1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240207F5

N° de cat : 83318-2-PBS

Synonymes

glypican 1, FLJ38078, glypican, glypican1, Glypican-1

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00342-2

  Sandwich ELISA standard curve of MP00342-2, human GPC1 Recombinant Matched Antibody Pair - PBS only. 83318-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0737. 83318-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00342-2

  Serum of fourteen individual healthy human donors was measured. The GPC1 concentration of detected samples was determined to be 21.8 ng/mL with a range of 10.3 - 32.1 ng/mL.

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  Sample test of MP00342-2

  Urine of eight individual healthy human donors was measured. The GPC1 concentration of detected samples was determined to be 3.3 ng/mL with a range of 0.6 - 7.2 ng/mL.

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  Sample test of MP00342-2

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human GPC1, and measured 13.1 ng/mL. HepG2 human hepatocellular carcinoma cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human GPC1, and measured 7.1 ng/mL.

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  Sample test of MP00342-2

  The mean GPC1 concentration was determined to be 10.0 ng/mL in MDA-MB-468 cell extract based on a 3.2 mg/mL extract load and 19.7 ng/mL in placenta tissue extract based on a 1.8 mg/mL extract load.

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  Cytometric bead array standard curve of MP00342-2

  Cytometric bead array standard curve of MP00342-2, GPC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83318-3-PBS. Detection antibody: 83318-2-PBS. Standard: Eg0737. Range: 1.25-40 ng/mL.

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Informations sur le produit

83318-2-PBS cible GPC1 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPC1 Protéine recombinante Eg0737
• Nom complet: glypican 1
• Masse moléculaire calculée: 558 aa, 62 kDa
• Numéro d’acquisition GenBank: BC051279
• Symbole du gène: GPC1
• Identification du gène (NCBI): 2817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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