Anticorps Recombinant de lapin anti-GPC1

GPC1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240207G5

N° de cat : 83318-4-RR

Synonymes

glypican 1, Glypican-1, glypican1, glypican, FLJ38078

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 83318-4-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 83318-4-RR (GPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83318-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83318-4-RR against Human GPC1 were performed. The affinity constant is 3.58 nM.

---

Applications testées

• Résultats positifs en WB: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83318-4-RR cible GPC1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: glypican 1
• Masse moléculaire calculée: 558 aa, 62 kDa
• Poids moléculaire observé: 61-65 kDa
• Numéro d’acquisition GenBank: BC051279
• Symbole du gène: GPC1
• Identification du gène (NCBI): 2817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glypicans are a family of HSPGs that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. Glypicans are considered to have the ability to modulate the activities of HBGFs. The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.

{{ptg:RelatedPrimaryAntibodies}}