Anticorps Recombinant de lapin anti-Glypican 2

Glypican 2 Recombinant Antibody for Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230518A4

N° de cat : 83241-1-PBS

Synonymes

GPC2, 230518A4, FLJ38962, Secreted glypican-2

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Galerie de données de validation

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  FC experiment of HeLa using 83241-1-RR (same clone as 83241-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83241-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 83241-1-RR (same clone as 83241-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83241-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00156-2

  Cytometric bead array standard curve of MP00156-2, Glypican 2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83241-1-PBS. Detection antibody: 83241-2-PBS. Standard: Ag4041. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 83241-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83241-1-RR against Human Glypican 2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83241-1-PBS cible Glypican 2 dans les applications de Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Glypican 2 Protéine recombinante Ag4041
• Nom complet: glypican 2
• Masse moléculaire calculée: 579 aa, 63 kDa
• Poids moléculaire observé: 62，70-100 kDa
• Numéro d’acquisition GenBank: BC027972
• Symbole du gène: GPC2
• Identification du gène (NCBI): 221914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).

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