Anticorps Polyclonal de lapin anti-Glypican 3

Glypican 3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30021-1-PBS

Synonymes

GPC3, DGSX, Glypican-3 alpha subunit, Glypican-3 beta subunit, GTR2 2

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Galerie de données de validation

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  WB analysis using 30021-1-AP (same clone as 30021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30021-1-AP (GPC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30021-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular carcinoma using 30021-1-AP (same clone as 30021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma slide using 30021-1-AP (Glypican 3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30021-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30021-1-PBS cible Glypican 3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glypican 3 Protéine recombinante Ag32559
• Nom complet: glypican 3
• Masse moléculaire calculée: 580 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC035972
• Symbole du gène: GPC3
• Identification du gène (NCBI): 2719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, and glycinate forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).

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