Anticorps Polyclonal de lapin anti-Glypican 4

• Phare
• Validé par KD/KO

Glypican 4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (2)

N° de cat : 13048-1-AP

Synonymes

Glypican 4, Glypican 4/6, GPC4, GPC4/6, K glypican

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Galerie de données de validation

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  WB analysis of A549 using 13048-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13048-1-AP (Glypican 4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 13048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13048-1-AP (Glypican 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13048-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13048-1-AP (Glypican 4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13048-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13048-1-AP (Glypican 4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

13048-1-AP cible Glypican 4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glypican 4 Protéine recombinante Ag3670
• Nom complet: glypican 4
• Masse moléculaire calculée: 556 aa, 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC017166
• Symbole du gène: GPC4
• Identification du gène (NCBI): 2239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation (PMID: 9931407). In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus (PMID: 10585884). They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Human GPC4 is a 556-amino acid protein, and shows 93.5% identity to mouse K-glypican. The gene of GPC4 has been mapped to Xq26, and its mRNA is widely expressed in human tissues, including lung, kidney, heart, placenta, skeletal muscle, and pancreas. GPC4 has also been shown to be present in astrocytes, haematopoietic-progenitor and bone-marrow-stromal cells. Studies suggest that GPC4 is involved in the regulation of Wnt signaling and astrocyte GPC4 plays a role in formation of excitatory synapses (PMID: 22302992; 22722203). This antobody can recognize both full length (65 kDa) and cleaved (35 kDa) GPC4 as well as GPC6 (PMID: 18055828; 22722203).

Publications

Species	Application	Title
mouse	WB	Nature; Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors.; Authors - Allen Nicola J NJ ; KO Validated
mouse	WB	Neuron; LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.; Authors - Reiko T Roppongi
human	IF	Neuron; Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation.; Authors - Isabella Farhy-Tselnicker
mouse	WB	Sci Adv; Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens.; Authors - Gajanan P Shelkar
mouse	WB,IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu ; KD Validated
human	IHC,IF	Nat Commun; CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.; Authors - Yuan Fang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rebecca (Verified Customer) (02-21-2022)

Western performed on Biorad MiniPROTEAN 4-15% TGX precast gels and Precision Plus All Blue Protein Ladder and Non-boil reducing buffer. Blocking performed for 1h at room temperature using 5%BLOTTO. Primary antibody dilution was performed overnight at 4°C in 5%BLOTTO/0.05%Tween. Secondary antibody was LiCOR IRDye® 680RD Donkey anti-Mouse IgG (H + L) at 1:20000 dilution in PBS-Tween-SDS as per manufacturers instructions for 2h at room temperature. Blot showed a clear band of the expected size with very low-none background/off target binding in the membrane fraction. Non-specific bands were observed in the cytosolic fraction.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat brain corticol lysates

Glypican 4 Antibody Western Blot validation (1:1000 dilution) in Rat brain corticol lysates (Cat no:13048-1-AP)

FH

Elena (Verified Customer) (11-19-2018)

• Applications: Western Blot,
• Cell Tissue Type: Brain

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