Anticorps Recombinant de lapin anti-GPD1

GPD1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242359D3

N° de cat : 84800-1-PBS

Synonymes

242359D3, cGPDH, EC:1.1.1.8, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, GPD 1

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Galerie de données de validation

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  WB analysis using 84800-1-RR (same clone as 84800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84800-1-RR (GPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84800-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84800-1-RR (same clone as 84800-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPD1 antibody (84800-1-RR, Clone: 242359D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84800-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84800-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84800-1-RR against Human GPD1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84800-1-PBS cible GPD1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPD1 Protéine recombinante Ag4278
• Nom complet: glycerol-3-phosphate dehydrogenase 1 (soluble)
• Masse moléculaire calculée: 349 aa, 38 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC032234
• Symbole du gène: GPD1
• Identification du gène (NCBI): 2819
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.

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