Anticorps Monoclonal anti-GPD2

GPD2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C12E2

N° de cat : 68174-1-PBS

Synonymes

1C12E2, EC:1.1.5.3, GDH2, Glycerol-3-phosphate dehydrogenase, mitochondrial, GPD 2

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Galerie de données de validation

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  WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68174-1-Ig (same clone as 68174-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50432-1

  Cytometric bead array standard curve of MP50432-1, GPD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68174-2-PBS. Detection antibody: 68174-1-PBS. Standard:Ag11212. Range: 0.391-200 ng/mL.

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  Affinity and Kinetic Characterization of 68174-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 68174-1-Ig against Human GPD2 was performed. The affinity constant is 6.26 nM.

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Informations sur le produit

68174-1-PBS cible GPD2 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPD2 Protéine recombinante Ag11212
• Nom complet: glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Masse moléculaire calculée: 41 kDa, 81 kDa
• Numéro d’acquisition GenBank: BC019874
• Symbole du gène: GPD2
• Identification du gène (NCBI): 2820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).

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