Anticorps Monoclonal anti-GPHN

GPHN Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D7C4

N° de cat : 67995-1-PBS

Synonymes

Gephyrin, Domain E, Domain G, GEPH, GPH

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Galerie de données de validation

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  WB analysis using 67995-1-Ig (same clone as 67995-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67995-1-Ig (GPHN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67995-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67995-1-Ig (same clone as 67995-1-PBS)

  WB result of GPHN antibody (67995-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPHN transfected HEK-293 cells. This data was developed using the same antibody clone with 67995-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67995-1-PBS cible GPHN dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPHN Protéine recombinante Ag23708
• Nom complet: gephyrin
• Masse moléculaire calculée: 769 aa, 83 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: BC030016
• Symbole du gène: GPHN
• Identification du gène (NCBI): 10243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.

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