Anticorps Polyclonal de lapin anti-GPI

• Phare
• Validé par KD/KO

GPI Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (2)

N° de cat : 15171-1-AP

Synonymes

AMF, Autocrine motility factor, g6pi, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36

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Galerie de données de validation

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  WB analysis of HeLa using 15171-1-AP

  WB result of GPI antibody (15171-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

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  WB analysis using 15171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15171-1-AP

  WB result of GPI antibody (15171-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

• 

  WB analysis of PC-3 using 15171-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15171-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15171-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 15171-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15171-1-AP (GPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC3, cellules PC-3, cellules U251, cellules U87-MG
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15171-1-AP cible GPI dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPI Protéine recombinante Ag7423
• Nom complet: glucose phosphate isomerase
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 55-64 kDa
• Numéro d’acquisition GenBank: BC004982
• Symbole du gène: GPI
• Identification du gène (NCBI): 2821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).

Publications

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
human	WB	EMBO Rep; OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.; Authors - Zhida Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (07-26-2021)

GPI is shown in green. Nuclei are shown in blue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RCC4

GPI Antibody Immunofluorescence validation (1:200 dilution) in RCC4 (Cat no:15171-1-AP)

FH

Daniel (Verified Customer) (02-06-2020)

Denatured lysate was separated on a 4-20% PAGE

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse kidney

GPI Antibody Western Blot, validation (1:2000 dilution) in mouse kidney (Cat no:15171-1-AP)