Anticorps Monoclonal anti-GPNMB

GPNMB Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

CloneNo.

2B10B8

Avis (1)

N° de cat : 66926-1-Ig

Synonymes

2B10B8, Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB

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Galerie de données de validation

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  WB analysis using 66926-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66926-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66926-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 66926-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (66926-1-Ig, Clone: 2B10B8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: SK-BR-3 cells, cellules 4T1, cellules A375, cellules A549, cellules HeLa, cellules HT-1080, cellules MDA-MB-468, cellules PC-12, cellules RAW 264.7, cellules THP-1, cellules U-251, cellules U-937
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Informations sur le produit

66926-1-Ig cible GPNMB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPNMB Protéine recombinante Ag26747
• Nom complet: glycoprotein (transmembrane) nmb
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC011595
• Symbole du gène: GPNMB
• Identification du gène (NCBI): 10457
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

Publications

Species	Application	Title
human	WB,IHC,IF	Int Immunopharmacol; Lactic acid-induced M2-like macrophages facilitate tumor cell migration and invasion via the GPNMB/CD44 axis in oral squamous cell carcinoma; Authors - Ying Lin
	WB	PLoS Negl Trop Dis; Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach; Authors - Yajie Yuan
human		medRxiv; Plasticity of Human Microglia and Brain Perivascular Macrophages in Aging and Alzheimer's Disease; Authors - Donghoon Lee
mouse	WB	Hum Vaccin Immunother; Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction; Authors - Kexin Zhang
human	IF	Immunity; Direct microglia replacement reveals pathologic and therapeutic contributions of brain macrophages to a monogenic neurological disease; Authors - William H Aisenberg
human	IHC	Cancers (Basel); TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma; Authors - Soji Toda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Victor (Verified Customer) (06-16-2025)

Staining with the GPNMB antibody on myeloid cells using the recommended concentration for immunofluorescence is highly specific. The signal shows minimal background noise and appears very clear and distinct.

• Applications: Western Blot, Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:500 as recommended
• Cell Tissue Type: Macrophages

GPNMB Antibody Western Blot,Immunofluorescence,Cell culture validation (1:500 as recommended dilution) in Macrophages (Cat no:66926-1-Ig)

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