Anticorps Recombinant de lapin anti-GPNMB

GPNMB Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241435F3

N° de cat : 84160-3-RR

Synonymes

241435F3, Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB

---

Galerie de données de validation

• 

  WB analysis using 84160-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84160-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 84160-3-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 84160-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (84160-3-RR, Clone: 241435F3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84160-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84160-3-RR against Human GPNMB were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules A375, cellules THP-1
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84160-3-RR cible GPNMB dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPNMB Protéine recombinante Ag14124
• Nom complet: glycoprotein (transmembrane) nmb
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 120 kDa, 95 kDa
• Numéro d’acquisition GenBank: BC011595
• Symbole du gène: GPNMB
• Identification du gène (NCBI): 10457
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

{{ptg:RelatedPrimaryAntibodies}}