Anticorps Polyclonal de lapin anti-GPR101-Specific

GPR101-Specific Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20074-1-PBS

Synonymes

GPR101, Probable G-protein coupled receptor 101

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Galerie de données de validation

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  WB analysis of HEK-293 using 20074-1-AP (same clone as 20074-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  WB analysis of COS-7 using 20074-1-AP (same clone as 20074-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 20074-1-AP (same clone as 20074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 20074-1-AP (same clone as 20074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 20074-1-AP (same clone as 20074-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human GPR101-Specific (20074-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20074-1-PBS cible GPR101-Specific dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, singe

• Réactivité: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: G protein-coupled receptor 101
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: NM_054021
• Symbole du gène: GPR101
• Identification du gène (NCBI): 83550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101.

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