Anticorps Polyclonal de lapin anti-GPR126

• Phare
• Validé par KD/KO

GPR126 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 17774-1-AP

Synonymes

ADGRG6, APG1, DREG, G protein coupled receptor 126, GPR126, PS1TP2, VIGR

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Galerie de données de validation

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  IHC staining of mouse kidney using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17774-1-AP (GPR126 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu rénal de souris, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

17774-1-AP cible GPR126 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPR126 Protéine recombinante Ag12195
• Nom complet: G protein-coupled receptor 126
• Masse moléculaire calculée: 1222 aa, 137 kDa
• Numéro d’acquisition GenBank: BC075798
• Symbole du gène: GPR126
• Identification du gène (NCBI): 57211
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPR126 (G protein-coupled receptor 126) is a member of the adhesion GPCR family. GPR126 is all widely expressed on stromal cells (PMID: 25713288). It is essential for normal differentiation of promyelinating Schwann cells and for normal myelination of axons (PMID: 24227709). GPR126 regulates neural, cardiac and ear development by G-protein- and/or N-terminus-dependent signaling (PMID: 24082093, PMID: 24067352). GPR126 also stimulates VEGF signaling and angiogenesis by modulating VEGF receptor 2 expression through STAT5 and GATA2 in endothelial cells.

Publications

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A; Progesterone activates GPR126 to promote breast cancer development via the Gi pathway.; Authors - Wentao An ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-04-2021)

calculated size is 130 kd. this antibody recognized major bands at 65, 55 kd.

• Applications: Western Blot

FH

Fiona (Verified Customer) (01-09-2020)

Immunofluorescence staining on A549 cells to look at expression. Staining was clean with little background, however some some very bright artefacts.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells