Anticorps Polyclonal de lapin anti-GPR161

GPR161 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 29328-1-AP

Synonymes

FLJ33952, G protein coupled receptor 161, G protein coupled receptor RE2, GPR161, RE2

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Galerie de données de validation

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  WB analysis using 29328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29328-1-AP (GPR161 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of ARPE-19 using 29328-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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  IF Staining of hTERT-RPE1 using 29328-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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  IF Staining of ARPE-19 using 29328-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IF/ICC: ARPE-19 cells, cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29328-1-AP cible GPR161 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPR161 Protéine recombinante Ag30998
• Nom complet: G protein-coupled receptor 161
• Masse moléculaire calculée: 529 aa, 59 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC028163
• Symbole du gène: GPR161
• Identification du gène (NCBI): 23432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band detected by this antibody probably represents a modified version of GPR161 (PMID: 18250320).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-25-2024)

I initially used GPR161 #13398-1-AP but it didn't worked at all in our NIH-3T3 nor in RPE1, nor in IMCD3 cells. So the company suggested this one and it worked nicely in IMCD3 (cf images GPR161 in green, ARL13B in red). It works a little less nicely in NIH-3T3 cells. I followed this protocol : remove medium add PFA 4% leave it 10 min wash 1xPBS add methanol in the freezer for 5min wash 2xPBS add blocking solution (1%BSA in PBST) leave it 1 hour add primary antibodies 1/400 wash 5x5min add secondary antibodies 1/500 wash 5x5min leave it in PBS image

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: IMCD3

GPR161 Antibody Immunofluorescence validation (1/400 dilution) in IMCD3 (Cat no:29328-1-AP)

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