Anticorps Polyclonal de lapin anti-GPR172B

GPR172B Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20791-1-PBS

Synonymes

RFT1, Protein GPR172B, PERV A receptor 2, PAR2, hRFT1

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Galerie de données de validation

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  WB analysis using 20791-1-AP (same clone as 20791-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20791-1-AP (GPR172B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20791-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 20791-1-AP (same clone as 20791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20791-1-AP (GPR172B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20791-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20791-1-PBS cible GPR172B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPR172B Protéine recombinante Ag14525
• Nom complet: G protein-coupled receptor 172B
• Masse moléculaire calculée: 448 aa, 46 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC060810
• Symbole du gène: GPR172B
• Identification du gène (NCBI): 55065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPR172B, also known as solute carrier family 52 member 1 (SLC52A1) or riboflavin transporter 1 (RFT1), is a G protein-coupled receptor (GPCR) that plays a significant role in the transport of riboflavin, a vital component for the production of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). GPR172B plays a role in cellular aging. Knockdown of GPR172B promotes senescence induced by DNA damage in both tumor and normal cells. The anti-senescence effect of GPR172B is dependent on its riboflavin transport activity, which leads to the activation of mitochondrial membrane potential (MMP) mediated by mitochondrial electron transport chain complex II, ultimately inhibiting the AMPK-p53 pathway, a central mediator of senescence-associated with mitochondrial dysfunction.

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