Anticorps Polyclonal de lapin anti-GPR35

• Phare
• Validé par KD/KO

GPR35 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 55248-1-AP

Synonymes

G protein coupled receptor 35, GPR35, KYNA receptor, Kynurenic acid receptor

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Galerie de données de validation

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  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55248-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 55248-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 55248-1-AP

  1X10^6 A549 cells were stained with 0.2ug GPR35 antibody (55248-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A549, tissu cérébral humain, tissu d'estomac humain
• Résultats positifs en cytométrie: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

55248-1-AP cible GPR35 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: G protein-coupled receptor 35
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: NM_005301
• Symbole du gène: GPR35
• Identification du gène (NCBI): 2859
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPR35, also named as KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.

Publications

Species	Application	Title
human	WB	Acta Pharm Sin B; Functional metabolomics reveal the role of AHR/GPR35 mediated kynurenic acid gradient sensing in chemotherapy-induced intestinal damage.; Authors - Di Wang ; KD Validated
human	WB	Eur J Pharmacol; Aminosalicylates target GPR35, partly contributing to the prevention of DSS-induced colitis; Authors - Wuxiyar Otkur