Anticorps Polyclonal de lapin anti-GPR35

• Phare
• Validé par KD/KO

GPR35 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 55248-1-AP

Synonymes

Kynurenic acid receptor, KYNA receptor, G-protein coupled receptor 35, G protein coupled receptor 35

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Galerie de données de validation

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  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55248-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 55248-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 55248-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55248-1-AP (GPR35 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55248-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55248-1-AP (GPR35 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu cérébral humain, tissu d'estomac humain
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

55248-1-AP cible GPR35 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: G protein-coupled receptor 35
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: NM_005301
• Symbole du gène: GPR35
• Identification du gène (NCBI): 2859
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPR35, also named the KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.

Publications

Species	Application	Title
human	WB	Acta Pharm Sin B; Functional metabolomics reveal the role of AHR/GPR35 mediated kynurenic acid gradient sensing in chemotherapy-induced intestinal damage.; Authors - Di Wang ; KD Validated
human	WB	Eur J Pharmacol; Aminosalicylates target GPR35, partly contributing to the prevention of DSS-induced colitis; Authors - Wuxiyar Otkur
human	WB	Science; Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists; Authors - Gregory A Wyant
mouse	IF	Microbiol Spectr; Prognostic implications of the interaction between intratumoral microbiome and immune response in gastric cancer; Authors - Sifan Liu

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