Anticorps Polyclonal de lapin anti-GPR81

• Phare
• Validé par KD/KO

GPR81 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20146-1-PBS

Synonymes

FKSG80, G protein coupled receptor 104, G protein coupled receptor 81, GPR104, G-protein coupled receptor 104

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Galerie de données de validation

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  WB analysis using 20146-1-AP (same clone as 20146-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20146-1-AP (GPR81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20146-1-PBS cible GPR81 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPR81 Protéine recombinante Ag13682
• Nom complet: G protein-coupled receptor 81
• Masse moléculaire calculée: 346 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC066881
• Symbole du gène: GPR81
• Identification du gène (NCBI): 27198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPR81 (G protein-coupled receptor 81) is one of a large family of GPRs with low affinity for hydroxy-carboxylic acid structure ligands (PMID: 24657625). Lactate functions as a signaling molecule by serving as an agonist for the GPR81, involving both autocrine and paracrine mechanisms (PMID: 31836453). Moreover, A higher molecular mass band (∼60 kDa) is present in the brain and adipose tissue. This band is also strong in the transfected HeLa cells but weak in native HeLa cells and is therefore probably a form of GPR81, possibly a glycosylated form of the receptor (PMID: 23696276).

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