Anticorps Polyclonal de lapin anti-GPSM1

• Phare
• Validé par KD/KO

GPSM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11483-1-AP

Synonymes

AGS3, GPSM1

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Galerie de données de validation

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  WB analysis of HeLa using 11483-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11483-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11483-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11483-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11483-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 11483-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11483-1-AP (GPSM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 11483-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11483-1-AP (GPSM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11483-1-AP

  Immunofluorescent analysis of HepG2 cells, using GPSM1 antibody 11483-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de souris, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

11483-1-AP cible GPSM1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPSM1 Protéine recombinante Ag2018
• Nom complet: G-protein signaling modulator 1 (AGS3-like, C. elegans)
• Masse moléculaire calculée: 652 aa, 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC017353
• Symbole du gène: GPSM1
• Identification du gène (NCBI): 26086
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).

Publications

Species	Application	Title
mouse	WB,IHC,IF	Nat Commun; GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages; Authors - Jing Yan
rat	WB,IHC	J Ovarian Res; Depletion of GPSM1 enhances ovarian granulosa cell apoptosis via cAMP-PKA-CREB pathway in vitro.; Authors - Xuzi Cai ; KD Validated
mouse	WB,IF	Mol Metab; GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis and provokes diet-induced obesity; Authors - Mengyang Tang