Anticorps Polyclonal de lapin anti-GPSM2

GPSM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 26798-1-AP

Synonymes

GPSM2, LGN, Mosaic protein LGN, Pins

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Galerie de données de validation

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  WB analysis using 26798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26798-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26798-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26798-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26798-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 26798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 26798-1-AP (GPSM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HEK-293T, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

26798-1-AP cible GPSM2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPSM2 Protéine recombinante Ag25195
• Nom complet: G-protein signaling modulator 2 (AGS3-like, C. elegans)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70-77 kDa
• Numéro d’acquisition GenBank: BC027732
• Symbole du gène: GPSM2
• Identification du gène (NCBI): 29899
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPSM2 belongs to a family of proteins that modulate activation of G proteins. GPSM2 assists in the exchange of guanine nucleotides, and allows extracellular signals to be transmitted to cells via cell surface, and ultimately plays a key role in the activation of G-proteins. Therefore, GPSM2 is a critical factor for the stability of cell division. Some recent studies have shown that GPSM2 messenger RNA (mRNA) is overexpressed and plays a positive role in the development of certain tumors, such as liver cancer, pancreatic cancer, breast cancer. It also plays a role in neuroblast division and in the development of normal hearing. Mutations in GPSM2 are associated with autosomal recessive nonsyndromic deafness (DFNB82), which is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; Comprehensive Analysis of GPSM2: From Pan-Cancer Analysis to Experimental Validation; Authors - Chunjiao Yang
human	IHC	Nan Fang Yi Ke Da Xue Xue Bao; [GPSM2 is highly expressed in gastric cancer to affect patient prognosis by promoting tumor cell proliferation]; Authors - Xue Song

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