Anticorps Monoclonal anti-GPT/ALT1

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5F6B5

N° de cat : 67531-1-PBS

Synonymes

ALAT1, GPT, GPT/ ALT1, AAT1, Alanine aminotransferase 1

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Galerie de données de validation

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  WB analysis of rat liver using 67531-1-Ig (same clone as 67531-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67531-1-Ig (same clone as 67531-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 67531-1-Ig (same clone as 67531-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67531-1-Ig (same clone as 67531-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 67531-1-Ig (same clone as 67531-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67531-1-Ig (same clone as 67531-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67531-1-PBS cible GPT/ALT1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPT/ALT1 Protéine recombinante Ag10453
• Nom complet: glutamic-pyruvate transaminase (alanine aminotransferase)
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC018207
• Symbole du gène: ALT1
• Identification du gène (NCBI): 2875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

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