Anticorps Polyclonal de lapin anti-GPX3

• Phare
• Validé par KD/KO

GPX3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13947-1-AP

Synonymes

Glutathione peroxidase 3, GPx 3, GPx P, GPX3, GPXP, GSHPx 3, GSHPx P, Plasma glutathione peroxidase

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Galerie de données de validation

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  WB analysis of mouse kidney using 13947-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13947-1-AP (GPX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat eye using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat eye using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 13947-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GPX3 antibody (13947-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris,
• Résultats positifs en IHC: tissu oculaire de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

13947-1-AP cible GPX3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPX3 Protéine recombinante Ag5054
• Nom complet: glutathione peroxidase 3 (plasma)
• Masse moléculaire calculée: 226 aa, 26 kDa
• Poids moléculaire observé: 20-27 kDa
• Numéro d’acquisition GenBank: BC050378
• Symbole du gène: GPX3
• Identification du gène (NCBI): 2878
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).

Publications

Species	Application	Title
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou
rat	WB	Oxid Med Cell Longev; The Secretome Deregulations in a Rat Model of Endotoxemic Shock.; Authors - A Blangy-Letheule
mouse	WB	Bioorg Chem; Two resveratrol analogs, pinosylvin and 4,4'-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway.; Authors - Cheng-Niu Wang
mouse	WB	Sci Rep; Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.; Authors - B Chellan
chicken	WB	J Cell Physiol; Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes.; Authors - Yafan Gong ; KD Validated