Anticorps Monoclonal anti-GPX4

• Phare
• Validé par KD/KO

GPX4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster et plus (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(697)

CloneNo.

3F5G5

Avis (5)

N° de cat : 67763-1-Ig

Synonymes

3F5G5, EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4

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Galerie de données de validation

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  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67763-1-Ig

  WB result of GPX4 antibody (67763-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells.

• 

  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 67763-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67763-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules EC109, cellules 4T1, cellules ATDC-5, cellules CHO, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MDCK, cellules NCCIT, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de poulet, tissu cérébral de rat, tissu cérébral de souris, tissu de poisson-zèbre, tissu plaquettaire humain, tissu testiculaire de lapin, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 633 publications below
• IHC: See 132 publications below
• IF: See 116 publications below
• IP: See 9 publications below
• CoIP: See 4 publications below

Informations sur le produit

67763-1-Ig cible GPX4 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster

• Réactivité: chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster
• Réactivité citée: rat, bovin, Chèvre, Humain, Lapin, porc, poulet, souris, duck
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPX4 Protéine recombinante Ag30650
• Nom complet: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC021567
• Symbole du gène: GPX4
• Identification du gène (NCBI): 2879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publications

Species	Application	Title
mouse	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
mouse,human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
rat	IF	Redox Biol; LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX; Authors - Dongya Jiang
mouse	WB	Redox Biol; A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease; Authors - Yi Sun
human	WB,IHC	Redox Biol; Targeting GPX4-mediated ferroptosis protection sensitizes BRCA1-deficient cancer cells to PARP inhibitors; Authors - Xuexia Xie
mouse	WB	Drug Des Devel Ther; Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides; Authors - Xuedong Yin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Pooja (Verified Customer) (09-08-2025)

mouse retina paraffin embedded section incubated with the GPx4 antibody (1:100 dilution) at 4 degree overnight.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse retina

GPX4 Antibody Immunohistochemistry validation (1:100 dilution) in Mouse retina (Cat no:67763-1-Ig)

FH

Breanna (Verified Customer) (08-11-2025)

The antibody worked well when testing my GPX4 KO cells! Highly recommend.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Rajeshkumar (Verified Customer) (07-23-2025)

works very good for human and mic lysates

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1-1000
• Cell Tissue Type: Human and mouse

FH

Vasu (Verified Customer) (09-23-2024)

We evaluated antibodies from various manufacturers but failed to achieve satisfactory results. However, using the ProteinTech antibody, we successfully detected robust and specific bands corresponding to the expected protein size.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast Cancer and other cancer cells and tumor tissue samples

FH

Tianyi (Verified Customer) (08-17-2023)

Paraffine-embedded ovarian tissue (4% PFA, 24 h) incubated with GPX4 (1:200 dilution) and secondary antibody Alexa fluor donkey anti-mouse 647 IgG (H+L).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Ovarian tissue

GPX4 Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:67763-1-Ig)

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