Anticorps Monoclonal anti-GPX4

• Phare
• Validé par KD/KO

GPX4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F5G5

N° de cat : 67763-1-PBS

Synonymes

3F5G5, EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4

---

Galerie de données de validation

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67763-1-Ig (same clone as 67763-1-PBS)

  WB result of GPX4 antibody (67763-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67763-1-PBS cible GPX4 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster

• Réactivité: chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPX4 Protéine recombinante Ag30650
• Nom complet: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC021567
• Symbole du gène: GPX4
• Identification du gène (NCBI): 2879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

{{ptg:RelatedPrimaryAntibodies}}