Anticorps Recombinant de lapin anti-GPX4 (Human Specific)

• Phare
• Validé par KD/KO

GPX4 (Human Specific) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

6E9

N° de cat : 82822-2-RR

Synonymes

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

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Galerie de données de validation

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  WB analysis using 82822-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82822-2-RR

  WB result of GPX4 (Human Specific) antibody (82822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 (Human Specific) transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 82822-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:15000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

82822-2-RR cible GPX4 (Human Specific) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPX4 (Human Specific) Protéine recombinante Ag30650
• Nom complet: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC021567
• Symbole du gène: GPX4
• Identification du gène (NCBI): 2879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; HDAC inhibitors activate lipid peroxidation and ferroptosis in gastric cancer; Authors - Robert Jenke
	WB	Viruses; Infectious Spleen and Kidney Necrosis Virus Triggers Ferroptosis in CPB Cells to Enhance Virus Replication; Authors - Qiushuang Zhang

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