Anticorps Polyclonal de lapin anti-GPX5

GPX5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18731-1-PBS

Synonymes

GSHPx 5, GPx-5, GPx 5, Epididymis-specific glutathione peroxidase-like protein, EGLP

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Galerie de données de validation

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  WB analysis of mouse testis using 18731-1-AP (same clone as 18731-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18731-1-AP (same clone as 18731-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 18731-1-AP (same clone as 18731-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 18731-1-AP (same clone as 18731-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18731-1-AP (same clone as 18731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18731-1-AP (same clone as 18731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18731-1-PBS cible GPX5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glutathione peroxidase 5 (epididymal androgen-related protein)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 22-28 kDa
• Numéro d’acquisition GenBank: NM_001509
• Symbole du gène: GPX5
• Identification du gène (NCBI): 2880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.

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