Anticorps Monoclonal anti-GRB2

• Phare
• Validé par KD/KO

GRB2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C5D11

N° de cat : 66880-1-PBS

Synonymes

1C5D11, ASH, EGFRBP GRB2, Grb33, Growth factor receptor-bound protein 2

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Galerie de données de validation

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  WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66880-1-Ig (same clone as 66880-1-PBS)

  WB result of GRB2 antibody (66880-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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  WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66880-1-PBS cible GRB2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GRB2 Protéine recombinante Ag0395
• Nom complet: growth factor receptor-bound protein 2
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC000631
• Symbole du gène: GRB2
• Identification du gène (NCBI): 2885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

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