Anticorps Polyclonal de lapin anti-GRB7

• Phare
• Validé par KD/KO

GRB7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10045-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10045-1-Ig (same clone as 10045-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  IP result of anti-GRB7 (IP:10045-1-Ig, 3ug; Detection:10045-1-Ig 1:500) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GRB7 antibody (10045-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10045-1-PBS cible GRB7 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRB7 Protéine recombinante Ag0023
• Nom complet: growth factor receptor-bound protein 7
• Masse moléculaire calculée: 532 aa, 60 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC006535
• Symbole du gène: GRB7
• Identification du gène (NCBI): 2886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.

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