Anticorps Polyclonal de lapin anti-GREB1

GREB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28699-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28699-1-AP (same clone as 28699-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 28699-1-AP (same clone as 28699-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28699-1-AP (same clone as 28699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28699-1-AP (same clone as 28699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28699-1-AP (same clone as 28699-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28699-1-AP (same clone as 28699-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28699-1-PBS cible GREB1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GREB1 Protéine recombinante Ag29908
• Nom complet: GREB1 protein
• Masse moléculaire calculée: 216 kDa
• Poids moléculaire observé: 216-240 kDa
• Numéro d’acquisition GenBank: NM_014668
• Symbole du gène: GREB1
• Identification du gène (NCBI): 9687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.

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