Anticorps Polyclonal de lapin anti-Glutamate receptor 2

Glutamate receptor 2 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (1)

N° de cat : 11994-1-AP

Synonymes

GluA2, GluR 2, GluR B, GluR K2, GLUR2, GLURB, Glutamate receptor 2, GRIA2, HBGR2

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Galerie de données de validation

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  WB analysis of mouse brain using 11994-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11994-1-AP

  IP Result of anti-Glutamate receptor 2 (IP:11994-1-AP, 4ug; Detection:11994-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 11994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 11994-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Glutamate receptor 2 antibody (11994-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 11994-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug Glutamate receptor 2 antibody (11994-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

• 

  FC experiment of SH-SY5Y using 11994-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Glutamate receptor 2 (11994-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu oculaire de souris,
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11994-1-AP cible Glutamate receptor 2 dans les applications de WB, IP, IHC, IF, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glutamate receptor 2 Protéine recombinante Ag2662
• Nom complet: glutamate receptor, ionotropic, AMPA 2
• Masse moléculaire calculée: 883 aa, 99 kDa
• Poids moléculaire observé: 94-105 kDa
• Numéro d’acquisition GenBank: BC010574
• Symbole du gène: GRIA2
• Identification du gène (NCBI): 2891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse,human	WB,CoIP	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
human	WB	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
mouse	WB	Mol Neurobiol; A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons.; Authors - Davide Capauto
mouse	IP	Cell Death Dis; ZDHHC8 critically regulates seizure susceptibility in epilepsy.; Authors - Qin Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is quite strong (50-70sec of exposure) and gives "clean" bands. The diluted antibody (TBS 0.1% Tween+5% BSA) does not work well anymore after 2-3 times of re-use

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons

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