Anticorps Polyclonal de lapin anti-Glutamate receptor 3/GluA3

Glutamate receptor 3/GluA3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 29588-1-AP

Synonymes

GRIA3, GluA3, GluR 3, GLUR C, GLUR K3

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Galerie de données de validation

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  WB analysis using 29588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29588-1-AP (GRIA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 29588-1-AP

  IP result of anti-GRIA3 (IP:29588-1-AP, 4ug; Detection:29588-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of mouse brain using 29588-1-AP

  IP result of anti-Glutamate receptor 3/GluA3 (IP:29588-1-AP, 4ug; Detection:29588-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 29588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 29588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamate receptor 3/GluA3 antibody (29588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 29588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Glutamate receptor 3/GluA3 antibody (29588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

29588-1-AP cible Glutamate receptor 3/GluA3 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glutamate receptor 3/GluA3 Protéine recombinante Ag30898
• Nom complet: glutamate receptor, ionotrophic, AMPA 3
• Masse moléculaire calculée: 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: NM_007325
• Symbole du gène: GRIA3
• Identification du gène (NCBI): 2892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA3 (Glutamate ionotropic receptor AMPA type subunit 3), also known as Glutamate receptor R3 or A3 (GluR3 or GluA3), is associated with X-linked intellectual disability (ID), dysmorphic features, and non-syndromic epilepsy(PMID: 32977175). GRIA3 also plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1(PMID: 20689760).

Publications

Species	Application	Title
mouse	WB	Cell Discov; Postsynaptic lncRNA Sera/Pkm2 pathway orchestrates the transition from social competition to rank by remodeling the neural ensemble in mPFC; Authors - Ling-Shuang Zhu
mouse	WB	Brain Res; Low-dose diazepam improves cognitive function in APP/PS1 mouse models: Involvement of AMPA receptors; Authors - Junwen Chen
mouse	IHC	iScience; The decreased astrocyte-microglia interaction reflects the early characteristics of Alzheimer's disease; Authors - Kefu Liu

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