Anticorps Polyclonal de lapin anti-GRID1

GRID1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 13040-1-AP

Synonymes

GluD1, GluR delta 1 subunit, GluR delta-1 subunit, Glutamate receptor ionotropic, delta-1, KIAA1220

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Galerie de données de validation

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  WB analysis of human brain using 13040-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13040-1-AP (GRID1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13040-1-AP

  IP result of anti-GRID1 (IP:13040-1-AP, 4ug; Detection:13040-1-AP 1:300) with mouse brain tissue lysate 5600ug.

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  IHC staining of human gliomas using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

13040-1-AP cible GRID1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRID1 Protéine recombinante Ag3694
• Nom complet: glutamate receptor, ionotropic, delta 1
• Masse moléculaire calculée: 1009 aa, 112 kDa
• Poids moléculaire observé: 100-112 kDa
• Numéro d’acquisition GenBank: BC039263
• Symbole du gène: GRID1
• Identification du gène (NCBI): 2894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRID1 (Glutamate Ionotropic Receptor Delta Type Subunit 1) is a protein-coding gene that belongs to the family of ionotropic glutamate receptors. These receptors are crucial for rapid excitatory synaptic transmission and synaptic plasticity in the central nervous system. GRID1 is primarily expressed in various brain regions, including the cortex, hippocampus, amygdala, striatum, thalamus, nucleus accumbens, lateral hooked nucleus, and dorsal raphe nucleus. In the hippocampus, GRID1 is specifically expressed in the granule cells of the dentate gyrus.

Publications

Species	Application	Title
mouse	IF	Anesthesiology; Single-nucleus Atlas of Sevoflurane-induced Hippocampal Cell Type- and Sex-specific Effects during Development in Mice; Authors - Shao-Yong Song

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