Anticorps Polyclonal de lapin anti-NMDAR1/GRIN1

NMDAR1/GRIN1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 27676-1-AP

Synonymes

GRIN1, NMDAR1, GluN1, Glutamate receptor ionotropic, NMDA 1, NMDAR 1

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Galerie de données de validation

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  WB analysis using 27676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 27676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27676-1-AP

  IP result of anti-NMDAR1/GRIN1 (IP:27676-1-AP, 4ug; Detection:27676-1-AP 1:2000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 27676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR1/GRIN1 antibody (27676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NMDAR1/GRIN1 antibody (27676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

27676-1-AP cible NMDAR1/GRIN1 dans les applications de WB, IHC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMDAR1/GRIN1 Protéine recombinante Ag26364
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_000832
• Symbole du gène: NMDAR1
• Identification du gène (NCBI): 2902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
rat	WB	Aging (Albany NY); EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.; Authors - Zhao-Hui Yao
mouse	WB	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
mouse	WB	J Neurosci; Cortex-specific Tmem169 Deficiency Induces Defects in Cortical Neuron Development and Autism-like Behaviors in Mice; Authors - Junhao Wang
mouse	WB	Exp Neurol; DPP-4 inhibition by linagliptin ameliorates age-related mild cognitive impairment by regulating microglia polarization in mice; Authors - Fen Zhuge
rat	WB,IP	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang

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