Anticorps Monoclonal anti-NMDAR2B/GRIN2B

NMDAR2B/GRIN2B Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1C5E12

Avis (1)

N° de cat : 66565-1-Ig

Synonymes

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

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Galerie de données de validation

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  WB analysis of mouse brain using 66565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66565-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66565-1-Ig cible NMDAR2B/GRIN2B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NMDAR2B/GRIN2B Protéine recombinante Ag16718
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Masse moléculaire calculée: 1484 aa, 166 kDa
• Poids moléculaire observé: 166 kDa
• Numéro d’acquisition GenBank: BC113620
• Symbole du gène: GRIN2B
• Identification du gène (NCBI): 2904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Publications

Species	Application	Title
rat	WB	Neuron; Structural basis for channel gating and blockade in tri-heteromeric GluN1-2B-2D NMDA receptor; Authors - Hyunook Kang
mouse	WB	Philos Trans R Soc Lond B Biol Sci; Cage effects on synaptic plasticity and its modulation in a mouse model of fragile X syndrome; Authors - Rasa Volianskis

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is not very strong and specific on hiPSCs derived neurons: it is detectable with an exposure of about 90 seconds, but sometimes gives aspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons

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