Anticorps Monoclonal anti-GRP75

GRP75 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B12F2

N° de cat : 67563-1-PBS

Synonymes

HSPA9, 2B12F2, 75 kDa glucose-regulated protein, CSA, GRP-75

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Galerie de données de validation

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  WB analysis using 67563-1-Ig (same clone as 67563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67563-1-Ig (GRP75 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67563-1-PBS cible GRP75 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GRP75 Protéine recombinante Ag7125
• Nom complet: heat shock 70kDa protein 9 (mortalin)
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC000478
• Symbole du gène: GRP75
• Identification du gène (NCBI): 3313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)

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