Anticorps Recombinant de lapin anti-GRP75

GRP75 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251670D1

N° de cat : 86721-1-RR

Synonymes

HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9

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Galerie de données de validation

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  WB analysis using 86721-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86721-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A549, cellules HT-29, cellules NIH/3T3, cellules SW480, tissu hépatique de souris, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86721-1-RR cible GRP75 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GRP75 Protéine recombinante Ag6673
• Nom complet: heat shock 70kDa protein 9 (mortalin)
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC000478
• Symbole du gène: GRP75
• Identification du gène (NCBI): 3313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)

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