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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-GRP78/BIP

GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 11587-1-AP

Synonymes

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSP A5, HSPA5, MIF2



Applications testées

Résultats positifs en WBcellules BxPC-3, cellules Caco-2, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HT-29, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
Résultats positifs en IPcellules MCF-7,
Résultats positifs en IHCtissu de cancer du pancréas humain, tissu cardiaque de souris, tissu cérébral de rat, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu rénal de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules HepG2,
Résultats positifs en cytométriecellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

11587-1-AP cible GRP78/BIP dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, poisson-zèbre, poulet, singe, souris, embryons de médaka, fish
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GRP78/BIP Protéine recombinante Ag2188
Nom complet heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Masse moléculaire calculée 78 kDa
Poids moléculaire observé 70-78 kDa
Numéro d’acquisition GenBankBC020235
Symbole du gène HSPA5
Identification du gène (NCBI) 3309
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Protocole

Product Specific Protocols
WB protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IHC protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IF protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IP protocol for GRP78/BIP antibody 11587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Science

Translation from the 5' untranslated region shapes the integrated stress response.

Authors - Shelley R Starck
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
mouseWB

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
human,ratWB

Acta Pharm Sin B

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

Authors - Fen Long
human,mouseWB

J Pineal Res

Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress

Authors - Zhou Du

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

PK (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: FIBROBLAST
GRP78/BIP Antibody Western Blot validation ( dilution) in FIBROBLAST (Cat no:11587-1-AP)
FH

Yeawon (Verified Customer) (03-17-2022)

Works very good in mouse kidney tissue.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse kidney tissue
FH

Emma (Verified Customer) (03-15-2022)

Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate
FH

P (Verified Customer) (05-27-2021)

Excellent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: H9C2
GRP78/BIP Antibody Western Blot validation (1:2000 dilution) in H9C2 (Cat no:11587-1-AP)
FH

Kevin (Verified Customer) (01-24-2019)

Works pretty good in HepG2 cells. One single band was observed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2
FH

Sylvain (Verified Customer) (12-03-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: colon cancer cell lines
FH

Natalie (Verified Customer) (11-20-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Rat Dorsal Root Ganglia
GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1-AP)