Anticorps Monoclonal anti-GRP78/BIP

• Phare
• Validé par KD/KO

GRP78/BIP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D6F7

N° de cat : 66574-1-PBS

Synonymes

BIP, GRP78, HSPA5, 1D6F7, 78 kDa glucose-regulated protein

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Galerie de données de validation

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  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

  IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed thapsigargin treated U2OS cells using GRP78/BIP antibody (66574-1-Ig, Clone: 1D6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66574-1-PBS cible GRP78/BIP dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GRP78/BIP Protéine recombinante Ag2188
• Nom complet: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 70-78 kDa
• Numéro d’acquisition GenBank: BC020235
• Symbole du gène: GRP78/BIP
• Identification du gène (NCBI): 3309
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

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