Anticorps Recombinant de lapin anti-GRP78,BIP

• Phare
• Validé par KD/KO

GRP78,BIP Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5F17

N° de cat : 80849-1-PBS

Synonymes

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

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Galerie de données de validation

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  WB analysis of HepG2 using 80849-1-RR (same clone as 80849-1-PBS)

  WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  WB analysis using 80849-1-RR (same clone as 80849-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80849-1-RR (same clone as 80849-1-PBS)

  IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80849-1-RR (same clone as 80849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 80849-1-RR (same clone as 80849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80849-1-RR (same clone as 80849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80849-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.

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Informations sur le produit

80849-1-PBS cible GRP78,BIP dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GRP78,BIP Protéine recombinante Ag2188
• Nom complet: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 70,78 kDa
• Numéro d’acquisition GenBank: BC020235
• Symbole du gène: GRP78/BIP
• Identification du gène (NCBI): 3309
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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