Anticorps Polyclonal de lapin anti-GRWD1

GRWD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 10354-1-AP

Synonymes

Glutamate-rich WD repeat-containing protein 1, GRWD, KIAA1942, RRB1, WDR28

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Galerie de données de validation

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10354-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 10354-1-AP

  IP result of anti-GRWD1 (IP:10354-1-AP, 4ug; Detection:10354-1-AP 1:1000) with PC-3 cells lysate 4000ug.

• 

  IHC staining of human prostate cancer using 10354-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10354-1-AP (GRWD1 antibody) at dilution of 1:100 (under 25x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules PC-3, cellules SKOV-3
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

10354-1-AP cible GRWD1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRWD1 Protéine recombinante Ag0367
• Nom complet: glutamate-rich WD repeat containing 1
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC002440
• Symbole du gène: GRWD1
• Identification du gène (NCBI): 83743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. (PMID:15885502)

Publications

Species	Application	Title
human	WB,IHC	Exp Cell Res; GRWD1 promotes cell proliferation and migration in non-small cell lung cancer by activating the Notch pathway.; Authors - Qiongzi Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zehua (Verified Customer) (12-17-2019)

I identified siRNAs targeting GRWD1 with this antibody. It works well for WB. I strongly suggested it.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7

GRWD1 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in MCF-7 (Cat no:10354-1-AP)

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