Anticorps Recombinant de lapin anti-GRWD1

GRWD1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251273F6

N° de cat : 86451-3-RR

Synonymes

Glutamate-rich WD repeat-containing protein 1, GRWD, KIAA1942, RRB1, WDR28

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Galerie de données de validation

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  WB analysis using 86451-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86451-3-RR (GRWD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86451-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 86451-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86451-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86451-3-RR against Human GRWD1 were performed. The affinity constant is 0.203 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules NIH/3T3, cellules PC-3, cellules SKOV-3
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86451-3-RR cible GRWD1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GRWD1 Protéine recombinante Ag0367
• Nom complet: glutamate-rich WD repeat containing 1
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49-63 kDa
• Numéro d’acquisition GenBank: BC002440
• Symbole du gène: GRWD1
• Identification du gène (NCBI): 83743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. (PMID:15885502)

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