Anticorps Polyclonal de lapin anti-GS28

GS28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 16106-1-AP

Synonymes

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16106-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16106-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16106-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16106-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 16106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach cancer using 16106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 16106-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16106-1-AP (GS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HepG2, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16106-1-AP cible GS28 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GS28 Protéine recombinante Ag9021
• Nom complet: golgi SNAP receptor complex member 1
• Masse moléculaire calculée: 28.6 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC012620
• Symbole du gène: GS28
• Identification du gène (NCBI): 9527
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-31-2025)

very good for WB

• Applications: Western Blot

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