Anticorps Recombinant de lapin anti-GS28

• Phare
• Validé par KD/KO

GS28 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242552G12

N° de cat : 84971-3-PBS

Synonymes

242552G12, 28 kDa cis-Golgi SNARE p28, 28 kDa Golgi SNARE protein, Golgi SNAP receptor complex member 1, GOS-28

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Galerie de données de validation

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  WB analysis using 84971-3-RR (same clone as 84971-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84971-3-RR (GS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84971-3-RR (same clone as 84971-3-PBS)

  WB result of GS28 antibody (84971-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GS28 transfected HepG2 cells. This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84971-3-RR (same clone as 84971-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84971-3-RR (same clone as 84971-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01719-2

  Cytometric bead array standard curve of MP01719-2, GS28 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84971-3-PBS. Detection antibody: 84971-4-PBS. Standard: Ag9021. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84971-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84971-3-RR against Human GS28 were performed. The affinity constant is 2.23 nM.

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Informations sur le produit

84971-3-PBS cible GS28 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GS28 Protéine recombinante Ag9021
• Nom complet: golgi SNAP receptor complex member 1
• Masse moléculaire calculée: 28.6 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC012620
• Symbole du gène: GS28
• Identification du gène (NCBI): 9527
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

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